Transformation Lab Report - Jane's AP Bio Webpage


transformation lab report

pGLO Transformation Lab Report Purpose: The purpose of this lab was to study transformation and the effect that integrating certain genes into a typical E. Coli bacteria would have on the cell. This lab also explored the effect that certain environments would have on the bacteria, including those containing antibiotics or certain sugar. pGLO TRANSFORMATION LAB REPORT. Introduction: The purpose of this lab was to observe the effects of the pGLO plasmid on various colonies of E. coli bacteria. pGLO is a genetically modified plasmid that primarily contains three genes (with the origin of replication).5/5(3). pGLO Transformation Lab Report. Lab report on the transformation of E. coli using pGLO plasmid DNA. Includes complete intr View more. University. California State University Los Angeles. Course. Principles of Biology I BIOL Academic year. 18//5(7).

Pglo Transformation Lab Report Essay |

Get access to this section to get all the help you need with your essay and educational goals. Transformation is the transfers of virulence from one cell to another, through the transferring of genetic material.

It was originally postulated in through the works of Federick Griffith, a British microbiologist, transformation lab report. Griffith observed that the mutant form, non-virulent form, of the bacteria Streptococcus Pnumoniae could transformation lab report transformed transformation lab report the normal, virulent form, when injected into mice along with heat killed normal forms.

He concluded that somehow the information the dead virulent form had transformed the mutant form into a virulent form. Later on through the works of Avery, transformation lab report, Macleod, and McCarty intransformation lab report, it became obvious that DNA is the transforming property and the substance transferred during transformation, between cells.

Furthermore, Hershey and Chase, inhypothesize that DNA and not protein is the genetic material in bacteriophages and after experimenting, concluded this theory and found that DNA must be the molecule used to reprogram cells.

DNA, shorthanded for Deoxyribonucleic acid is a nucleic acid contains instructions for the development, functionality, and maintenance of new cells. DNA consists as chains of nucleotides, in two transformation lab report strands in a double helix, connected by hydrogen bonds between complementary nitrogenous bases.

Segments of DNA carrying genetic information are called genes, which mostly code for a specific type of protein. This lab will focus on the gene pGLO, transformation lab report. Thus transformation becomes specifically expressed as the intake and influence of new genetic material in the form of DNA. The trait changes are often caused by the new genetic material causing a change in protein construction and composition.

This lab uses a gene called pGLO to transform fecal bacterium. The goal of the lab is to get the bacteria to intake and express transformation lab report pGLO gene and produce the protein, which will fluoresces green under the presence of ultraviolet light. Furthermore we will require plasmid in order to aid in the transformation process. Plasmids are additional circular units of genetic Material contained in bacteria and codes for trait that assist in bacterial survival, transformation lab report.

After bacterium growth on antibiotic plates, cells will appear in circular colonies, or as a strand of lawns, and if the transformation transformation lab report a success they will fluoresces green under ultraviolet light.

Before undergoing the transformation lab, transformation lab report, confirmation that the substance being added to the bacterium is DNA must be acquired. This is done through electrophoresis. This process creates a uniform electrical field that allows motion of particles of various sizes towards a positively charged end. A larger particles move slower in the charged viscous gel so we will compare water, a small molecule, to what we believe is a plasmid solution, macromolecule.

First, load the electrophoresis with algerose, Jell-O like solution that is liquid unless charged with an electrical current. Comb wells near the negative end of the electrophoresis and load samples of Plasmid Solution and DNA into the wells.

Before loading 10 microliters of each, the substance must be mixed with a loading dye on Para film to identify it in the charged algerose gel. Also add a 1 KB ladder marker to be able to determine size of plasmid molecules. As for the main experiment of transformation, start by looking at the same plasmid DNA solution under ultraviolent light to see if it fluoresces, transformation lab report.

Then obtain a solution of fecal bacterium, transformation lab report divide equally in two centrifuge tubes. Spin the two tubes in a centrifuge for 5 minutes on opposite side of the centrifuge. The bacterium will collect at the bottom of the tube, so pour out the extraneous supinate. Then, add microliters of buffer.

Place both tubes on ice. Next heat shocks the tubes for 50 seconds, followed by icing for 10 more minutes. The heat shock increases the permeability of the cell membrane to DNA. Then add milliliters of LB and incubate for 20 minutes.

The 20 minute incubation following the addition of LB broth allows the cells to grow and express the ampicillin resistance protein, beta-lactamase, so that the transformed cells survive the subsequent ampicillin selection plates, transformation lab report.

For the electrophoresis we see that plasmid DNA loaded in lane 3 migrated a lesser distance than the water loaded in lane 2, showing the plasmid DNA solution contained larger molecules. See picture below. When arabinose is present in the environment of the bacteria, it binds to araC and cause transcription and creation of the Green Fluoresce Protein by ribosomal units and tRNA. Ultraviolent light exposure is absorbed by the protein and slowly phosflouresces, emits absorbed light slowly while transforming some of it.

Part of the transformed phosflouresces is in the form of the green light we witness. Finally, transformation has man practical uses mostly in the area of biotechnology. Scientist can use the genes coding for a particular resistance, such transformation lab report B-lactamase, or an expression, such as arabinose, to inject in plants or animals in order to modify or introduce new traits. Another use of transformation is to create bacteria that can make insulin.

BP also used bacteria, transformed to consume oil, in cleaning up the gulf oil spill. And certain genes, transformation lab report, such as sickle cell anemia, are being eradicated in receptive humans by the use of gene therapy. This involves transforming the genes of a sick individual with healthy genes from a donor. Words: Category: Database Pages: 6. Get Full Essay Get access to this section to get all the help you need with your essay and educational goals.

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pglo transformation lab report | Transformation (Genetics) | Plasmid


transformation lab report


Genetic transformation is a process in which DNA is moved into an organism such that its genotype or genetic makeup is changed (What is, ). Though easier to accomplish on single-cell bacteria than multi-cellular organisms, this process allows us to change the traits of the original test organisms as well pass these changes to any future. Title: pGLO Transformation Lab Introduction: Genetic transformation is a change caused by genes, involving the insertion of a gene into. an organism to change the organisms trait. In this experiment, bacteria will be transformed with a gene that codes for Green Fluorescent Protein (GFP).5/5(17). ContentsMethodsDiscussion Introduction Transformation is the transfers of virulence from one cell to another, through the transferring of genetic material. It was originally postulated in through the works of Federick Griffith, a British microbiologist. Griffith observed that the mutant form, non-virulent form, of the bacteria Streptococcus Pnumoniae could be transformed into the normal.